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http://hdl.handle.net/10609/137147
Título : | Performance of an affordable urine self-sampling method for human papillomavirus detection in Mexican women |
Autoría: | Hernández López, Rubí Hermosillo, Luis León Maldonado, Leith Velázquez Cruz, Rafael Torres Ibarra, Leticia Lazcano Ponce, Eduardo Lörincz, Attila Wheeler, Cosette M. BOSCH JOSÉ, FRANCESC XAVIER Cuzick, Jack Rivera Paredez, Berenice Nedjai, Belinda Salmerón, Jorge |
Otros: | Universitat Oberta de Catalunya (UOC) |
Citación : | Hernández-López R, Hermosillo L, León-Maldonado L, Velázquez-Cruz R, Torres-Ibarra L, Lazcano-Ponce E, et al. (2021) Performance of an affordable urine self-sampling method for human papillomavirus detection in Mexican women. PLoS ONE 16(7): e0254946. https://doi.org/10.1371/journal.pone.0254946 |
Resumen : | Introduction: Urine self-sampling for human papillomavirus (HPV)-based cervical cancer screening is a non-invasive method that offers several logistical advantages and high acceptability, reducing barriers related to low screening coverage. This study developed and evaluated the performance of a low-cost urine self-sampling method for HPV-testing and explored the acceptability and feasibility of potential implementation of this alternative in routine screening. Methods: A series of sequential laboratory assays examined the impact of several pre-analytical conditions for obtaining DNA from urine and subsequent HPV detection. Initially, we assessed the effect of ethylaminediaminetetraacetic acid (EDTA) as a DNA preservative examining several variables including EDTA concentration, specimen storage temperature, time between urine collection and DNA extraction, and first-morning micturition versus convenience sample collection. We further evaluated the agreement of HPV-testing between urine and clinician-collected cervical samples among 95 women. Finally, we explored the costs of self-sampling supplies as well as the acceptability and feasibility of urine self-sampling among women and healthcare workers. Results: Our results revealed higher DNA concentrations were obtained when using a 40mM EDTA solution, storing specimens at 25°C and extracting DNA within 72 hrs. of urine collection, regardless of using first-morning micturition or a convenience sampling. We observed good agreement (Kappa = 0.72) between urine and clinician-collected cervical samples for HPV detection. Furthermore, urine self-sampling was an affordable method (USD 1.10), well accepted among cervical cancer screening users, healthcare workers, and decision-makers. Conclusion: These results suggest urine self-sampling is feasible and appropriate alternative for HPV-testing in HPV-based screening programs in lower-resource contexts. |
Palabras clave : | urine cervical cancer human papillomavirus DNA extraction specimen storage urination Mexico human papillomavirus infection |
DOI: | https://doi.org/10.1371/journal.pone.0254946 |
Tipo de documento: | info:eu-repo/semantics/article |
Versión del documento: | info:eu-repo/semantics/publishedVersion |
Fecha de publicación : | 2-jul-2021 |
Aparece en las colecciones: | Articles Articles cientÍfics |
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