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Title: Estudio comparativo de herramientas bioinformáticas para el análisis metagenómico sobre el microbioma humano
Author: Jiménez Ocón, Lidia
Director: Prados Carrasco, Ferran
Tutor: Paytuví Gallart, Andreu
Keywords: human microbiome
16S rRNA
Issue Date: 8-Jan-2020
Publisher: Universitat Oberta de Catalunya (UOC)
Abstract: The purpose of this final master's project has been to make a comparison with different softwares for taxonomic analysis in different microbiomes of the human body. The metagenomic study was carried out thanks to the amplicon sequencing method of the 16S rRNA gene, this is a marker par excellence characterized by its low rate of evolution. To make the study, three datasets corresponding to intestinal, vaginal and oral microbiome were created. The simulation was carried out with the Wgsim software with data from the Illumina MiSeq, carrying out a paired-end sequencing. The bioinformatic analysis was carried out with three of the most commonly used software, which are: Gaia, Qiime2 and Mothur. These last two are open source, so to use them, an Ubuntu virtual machine was used in Google Cloud. Gaia has been developed by the company Sequentia Biotech, so there was no need to create any code for the analysis. Once the taxonomic analysis was performed, the statistical analysis of the results was carried out. For this, parameters such as precision, recall and F-measure were evaluated.
Language: Spanish
Appears in Collections:Bachelor thesis, research projects, etc.

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