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http://hdl.handle.net/10609/120626
Title: 16SPyPrimer: un paquete en Python para el análisis de la cobertura de primers del gen 16S rRNA
Author: Vázquez González, Lara M.
Director: Carlos Balsa Castro
María José Carreira Nouche
Dorcas Orengo Ferriz
Tutor: Ferran Prados Carrasco
Keywords: Coverage, primers, Python
Issue Date: Jun-2020
Publisher: Universitat Oberta de Catalunya (UOC)
Abstract: In various areas such as microbiology, ecology, or agriculture, it is important and necessary to properly identify the bacterial species present in any sample. An example may be the association of a specific pathology in a human, animal or plant due to the presence or absence of any specific bacterial species. In many areas of research on bacterial diversity, it is necessary to be able to identify specific groups of bacteria and reject other groups for different taxonomic levels. The 16S rRNA gene has been used for many years as a phylogenetic marker for bacterial identification, being used as a diagnostic gene for taxonomic identification. Many pairs of primers are described in the literature that work adequately for the different conserved areas of the 16S rRNA gene. However, generic primers that do not allow the selection of bacterial families of interest are often used. The aim of this project is to develop a tool in Python, called 16SPyPrimer, that analyses the coverage of pairs of primers for specific sets of bacteria. This software will allow researchers to modify already known primers to find more specific ones according to their needs. This tool has extensive configurable options according to the user's criteria and returns multiple results files with the necessary information to carry out other analysis afterwards.
Language: Spanish
URI: http://hdl.handle.net/10609/120626
Appears in Collections:Bachelor thesis, research projects, etc.

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