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Title: | Estudio de genómica comparada de genes bacterianos relacionados con la ruta catabólica inferior del naftaleno: metabolismo del salicilato |
Author: | Galli Medina, Irene |
Tutor: | Pizarro Tobías, Paloma |
Others: | Perez-Navarro, Antoni |
Abstract: | The increased presence of xenobiotics in the environment has increased interest in studying the degradation of these compounds, including bioremediation. Within these xenobiotics, the group of polycyclic aromatic hydrocarbons should be highlighted, where naphthalene stands out for being the simplest compound and, therefore, the most studied. The degradation of naphthalene by bacteria is carried out in two phases, although in this case, the study will focus on the lower pathway that can be carried out by two pathways: the catechol pathway and the gentisate pathway. In this work we propose a comparative genomics study of selected genes of the catechol pathway (nahG, nahH and nahI), using Pseudomonas putida G7 as a model, and of the gentisate pathway (nagG, nagH, nagI and nagAa) from Ralstonia sp. U2. As a result, we obtain a phylogenetic tree for each gene mentioned and, in addition, the prediction and comparison of some selected operons. It has been observed that in the databases for the catechol route, the majority of strains belong to the Gammaproteobacteria class while for the gentisic acid route the Betaproteobacteria class stands out. Furthermore, a high identity of Cupriavidus necator U2 with the selected genes of Ralstonia sp. U2 has been observed (except for nagI) and a high identity of Pseudomonas sp. MC1 and Pseudomonas umsongensis GO16 with the genes and the sal operon of Pseudomonas putida G7. |
Keywords: | bioremediation catechol gentisato |
Document type: | info:eu-repo/semantics/masterThesis |
Issue Date: | Jun-2021 |
Publication license: | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
Appears in Collections: | Trabajos finales de carrera, trabajos de investigación, etc. |
Files in This Item:
File | Description | Size | Format | |
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igalliTFM0621memoria.pdf | Memoria del TFM | 1,17 MB | Adobe PDF | View/Open |
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