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Título : Performance of an affordable urine self-sampling method for human papillomavirus detection in Mexican women
Autoría: Hernández López, Rubí
Hermosillo, Luis
León Maldonado, Leith
Velázquez Cruz, Rafael
Torres Ibarra, Leticia
Lazcano Ponce, Eduardo
Lörincz, Attila
Wheeler, Cosette M.
BOSCH JOSÉ, FRANCESC XAVIER  
Cuzick, Jack
Rivera Paredez, Berenice
Nedjai, Belinda
Salmerón, Jorge  
Otros: Universitat Oberta de Catalunya (UOC)
Citación : Hernández-López R, Hermosillo L, León-Maldonado L, Velázquez-Cruz R, Torres-Ibarra L, Lazcano-Ponce E, et al. (2021) Performance of an affordable urine self-sampling method for human papillomavirus detection in Mexican women. PLoS ONE 16(7): e0254946. https://doi.org/10.1371/journal.pone.0254946
Resumen : Introduction: Urine self-sampling for human papillomavirus (HPV)-based cervical cancer screening is a non-invasive method that offers several logistical advantages and high acceptability, reducing barriers related to low screening coverage. This study developed and evaluated the performance of a low-cost urine self-sampling method for HPV-testing and explored the acceptability and feasibility of potential implementation of this alternative in routine screening. Methods: A series of sequential laboratory assays examined the impact of several pre-analytical conditions for obtaining DNA from urine and subsequent HPV detection. Initially, we assessed the effect of ethylaminediaminetetraacetic acid (EDTA) as a DNA preservative examining several variables including EDTA concentration, specimen storage temperature, time between urine collection and DNA extraction, and first-morning micturition versus convenience sample collection. We further evaluated the agreement of HPV-testing between urine and clinician-collected cervical samples among 95 women. Finally, we explored the costs of self-sampling supplies as well as the acceptability and feasibility of urine self-sampling among women and healthcare workers. Results: Our results revealed higher DNA concentrations were obtained when using a 40mM EDTA solution, storing specimens at 25°C and extracting DNA within 72 hrs. of urine collection, regardless of using first-morning micturition or a convenience sampling. We observed good agreement (Kappa = 0.72) between urine and clinician-collected cervical samples for HPV detection. Furthermore, urine self-sampling was an affordable method (USD 1.10), well accepted among cervical cancer screening users, healthcare workers, and decision-makers. Conclusion: These results suggest urine self-sampling is feasible and appropriate alternative for HPV-testing in HPV-based screening programs in lower-resource contexts.
Palabras clave : urine
cervical cancer
human papillomavirus
DNA extraction
specimen storage
urination
Mexico
human papillomavirus infection
DOI: https://doi.org/10.1371/journal.pone.0254946
Tipo de documento: info:eu-repo/semantics/article
Versión del documento: info:eu-repo/semantics/publishedVersion
Fecha de publicación : 2-jul-2021
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