Please use this identifier to cite or link to this item:
http://hdl.handle.net/10609/139030
Title: | Análisis filogenético de la óxido nitroso reductasa (nosZ): establecimiento del perfil de la proteína |
Author: | Segarra Jornet, Sandra |
Tutor: | Pizarro Tobías, Paloma |
Others: | Perez-Navarro, Antoni |
Abstract: | One of the main objectives of the present work is the phylogenetic analysis of nitrous oxide reductase, an enzyme responsible for the reduction of N2O to N2 during biological denitrification. The other main objective is to establish the profile of this protein. For this purpose, it has been necessary to create a database that includes a set of protein sequences from different microbiological organisms, by searching for homologues of the query sequence in BLAST. After the preparation of the sequences, a multiple alignment is carried out with the MEGA-X software, as well as the determination of the evolutionary model and the phylogenetic signal, the latter using TREE-PUZZLE. For the study of the taxonomic classification of organisms possessing the nosZ gene, a phylogenetic tree is prepared using the probabilistic method of maximum likelihood with bootstrapping. From this analysis we obtain the distinction of the organisms into two clades, according to whether they possess the "typical" or "atypical" gene. The last step is the preparation of the protein profile. Two have been prepared, one for each Clade, using the Phyre2 web server. The three-dimensional protein structures have been predicted by homology modelling, both with a 100% confidence level. With the results obtained, it is confirmed that it is possible to extend and complement the studies already done on nitrous oxide reductase using public databases, a fact that could mean an important step in the potential reduction of N2O emissions in the European Union emissions of N2O, a potent greenhouse gas. |
Keywords: | nosZ phylogenetic analysis greenhouse gases Bioinformatics -- TFM |
Document type: | info:eu-repo/semantics/masterThesis |
Issue Date: | Dec-2021 |
Publication license: | http://creativecommons.org/licenses/by-nc-nd/3.0/es |
Appears in Collections: | Trabajos finales de carrera, trabajos de investigación, etc. |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
ssegarrajornetTFM1221memoria.pdf | Memoria del TFM | 2,17 MB | Adobe PDF | View/Open |
Share:
This item is licensed under a Creative Commons License