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Title: Anàlisis de l'evolució del genoma de Physcomitrella patens
Author: Vendrell, Pol
Director: Josep Maria Casacuberta
Tutor: Roser Pratdesaba Moreno
Others: Universitat Oberta de Catalunya
Keywords: Elements transposables
Issue Date: 21-May-2017
Publisher: Universitat Oberta de Catalunya
Abstract: In this paper, we analyzed the Physcomitrella patens evolution. We carried out two approximations to study this evolution. On the one side, we analyzed the polymorphisms in two ecotypes: Villersexel and Reute through data obtained by Next Generation Sequencing. On the other side, we analyzed the evolution of P. patens through multiple resequencing from the original clone used to generate the annotation that had been vegetative propagated during the last 8 years. This has been possible due to some resequencing samples that come from other ones resequenced during that time. We examined these data using programs that detect polymorphism transposable elements which allow the analysis of sequencing using paired-end. We described the best methodology in order to detect polymorphism transposable elements and we obtained the results from the polymorphic elements in the ecotypes Villersexel and Reute. We verified that the RLG1 elements are the ones that present most movement in the Physcomitrella patens genome and that these elements tend to get inserted inside other transposable elements. Moreover, we confirmed that the Reute ecotype is highly similar to the Gransden ecotype (reference genome) and at the same time the Villersexel presents much more polymorphism, which means it is more distant to the reference. Finally, we were able to verify that with the available samples from the last 8 years it has not been possible to detect any polymorphic transposable element.
Language: Catalan
Appears in Collections:Bachelor thesis, research projects, etc.

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