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Title: Efecto del microbioma vaginal en la tasa de embarazo en pacientes que se someten a técnicas de reproducción asistida
Author: Lledó Bosch, Belen
Director: Pizarro Tobías, Paloma
Tutor: Merino Arranz, David
Others: Universitat Oberta de Catalunya
Keywords: assisted reproduction methods
in vitro fertilization
Issue Date: 1-Jun-2018
Publisher: Universitat Oberta de Catalunya
Abstract: The vaginal microbiome plays a very important role in the maintenance of the integral health of the woman, being comprised basically of bacteria of the genus Lactobacillus. Currently more and more couples are using assisted reproduction techniques (ART), which should be offered a multidisciplinary approach (gynecological, endocrine, genetic, immunological, infectious ... etc) for diagnosis. Recent evidence seems to indicate that there is a relationship between the vaginal microbiome and fertility, however, it is unknown whether this effect occurs when couples undergo ART. The aim of this work was to evaluate the impact of the vaginal microbiome day of embryo transfer on the pregnancy rate of patients undergoing in vitro fertilization treatment. To achieve this objective, vaginal samples from 31 patients who have undergone a transfer of chromosomally normal cryopreserved embryos have been analyzed. The analysis of the rRNA16S V3V4 region and the bioinformatic tools QIIME2, MicrobiomeAnalyst and Phyloseq have been used to determine the microbiome.From our results we conclude that the patients who achieve pregnancy have a lower diversity than the patients who do not achieve it. In addition, the presence of Lactobacillus seems to be key to embryo implantation. On the other hand, the presence of bacteria of the genus Gadnerella seems to have a negative influence on pregnancy. Moreover, the vaginal microbiome does not influence whether the patient suffers implantation failure or has not been treated with probiotics.
Language: Spanish
Appears in Collections:Bachelor thesis, research projects, etc.

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