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http://hdl.handle.net/10609/81829
Title: | Efecto del microbioma vaginal en la tasa de embarazo en pacientes que se someten a técnicas de reproducción asistida |
Author: | Lledó Bosch, Belen |
Tutor: | Pizarro Tobías, Paloma |
Others: | Universitat Oberta de Catalunya Merino, David |
Abstract: | The vaginal microbiome plays a very important role in the maintenance of the integral health of the woman, being comprised basically of bacteria of the genus Lactobacillus. Currently more and more couples are using assisted reproduction techniques (ART), which should be offered a multidisciplinary approach (gynecological, endocrine, genetic, immunological, infectious ... etc) for diagnosis. Recent evidence seems to indicate that there is a relationship between the vaginal microbiome and fertility, however, it is unknown whether this effect occurs when couples undergo ART. The aim of this work was to evaluate the impact of the vaginal microbiome day of embryo transfer on the pregnancy rate of patients undergoing in vitro fertilization treatment. To achieve this objective, vaginal samples from 31 patients who have undergone a transfer of chromosomally normal cryopreserved embryos have been analyzed. The analysis of the rRNA16S V3V4 region and the bioinformatic tools QIIME2, MicrobiomeAnalyst and Phyloseq have been used to determine the microbiome.From our results we conclude that the patients who achieve pregnancy have a lower diversity than the patients who do not achieve it. In addition, the presence of Lactobacillus seems to be key to embryo implantation. On the other hand, the presence of bacteria of the genus Gadnerella seems to have a negative influence on pregnancy. Moreover, the vaginal microbiome does not influence whether the patient suffers implantation failure or has not been treated with probiotics. |
Keywords: | assisted reproduction methods in vitro fertilization microbiome |
Document type: | info:eu-repo/semantics/masterThesis |
Issue Date: | 1-Jun-2018 |
Publication license: | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
Appears in Collections: | Trabajos finales de carrera, trabajos de investigación, etc. |
Files in This Item:
File | Description | Size | Format | |
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blledoTFM0618memoria.pdf | Memoria del TFM | 1,44 MB | Adobe PDF | View/Open |
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