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Title: Determinación de marcas epigenéticas específicas de tejido para la identificación del origen de las vesículas circulantes
Author: Martínez Micaelo, Neus
Director: Merino Arranz, David
Tutor: Brunel Montaner, Helena
Others: Universitat Oberta de Catalunya
Keywords: epigenetic
extracellular blisters
Issue Date: 5-Jun-2018
Publisher: Universitat Oberta de Catalunya
Abstract: The aim of this Final Master's Project (FMP) is to identify the origin of circulating extracellular vesicles. Taking into account that the extracellular vesicles contain genetic material from the progenitor cell, including genomic DNA, and that each cell type has a specific epigenetic pattern, the main objective of this FMP is the application of bioinformatic methods and tools on epigenetic data deposited in public repositories, for the design and development of an algorithm based on epigenetic marks to differentiate between tissues. To achieve this objective, the DNA methylation data from a total of 252 experiments, grouped in 24 tissuespecific or cell-type clusters, were analyzed and positions or loci were identified by creating a function (BiosourceMapper). Differentially methylated positions specific for the tissue or cell type selected, as well as regions enriched in differentially methylated positions, were found. Furthermore, to improve the visualization and interpretation of the data, the shiny Biosource Mapper App has been created, allowing users to access and analyze the results generated within this TFM. The main conclusion obtained is that bioinformatic analysis of the methylation pattern with the BiosourceMapper function implemented in the Biosource Mapper App identify specific tissue marks.
Language: Spanish
Appears in Collections:Bachelor thesis, research projects, etc.

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