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http://hdl.handle.net/10609/82265
Title: Validación de una aplicación para la anotación de isomiRs
Author: Alis Pozo, Rafael
Director: Pantano Rubiño, Lorena
Tutor: Marco Galindo, María Jesús
Keywords: microRNA
isomiR
bioinformatics
Issue Date: 5-Jun-2018
Publisher: Universitat Oberta de Catalunya (UOC)
Abstract: Micro RNAs (miRNA) are a class of non-coding small RNAs with a length around 22 nucleotides and a repressor function of gene expression. It has been shown that a single miRNA locus might yield several distinct sequences as result of the maturation processes. The small RNA with these variations in relation to the reference miRNA are called isomiRs. The determination of the expression levels of miRNA by massive Next Generation Sequencing (NGS) is very popular. However, sequence annotation is usually performed ignoring the miRNA isomers, in spite of been shown that the miRNA biological activity is regulated by the modifications in the sequence. In this work we have helped to implement a file format to annotate small RNA-seq data, consistent with the GFF3 standard. Moreover, we have developed bioinformatic tools to check that file format and import the data to Python environment. Lastly, we have checked the usefulness of the file format and the developed tools analyzing a set of experimental data.
Language: Spanish
URI: http://hdl.handle.net/10609/82265
Appears in Collections:Bachelor thesis, research projects, etc.

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