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dc.contributor.authorPaytubi, Sonia-
dc.contributor.authorTaberna , Miren-
dc.contributor.authorMena, Marisa-
dc.contributor.authorQuirós, Beatriz-
dc.contributor.authorGomà-i-Freixanet, Montserrat-
dc.contributor.authorAlemany, Laia-
dc.contributor.authorBOSCH JOSÉ, FRANCESC XAVIER-
dc.contributor.authorPAVÓN, MIGUEL ANGEL-
dc.contributor.otherInstitut Català d'Oncologia-
dc.contributor.otherInstitut d'Investigació Biomèdica de Bellvitge-
dc.contributor.otherInstituto de Salud Carlos III-
dc.contributor.otherHospital Universitari de Bellvitge-
dc.contributor.otherUniversitat Oberta de Catalunya-
dc.date.accessioned2022-08-09T06:58:06Z-
dc.date.available2022-08-09T06:58:06Z-
dc.date.issued2021-11-17-
dc.identifier.citationPaytubi, S., Taberna, M., Quirós, B., Gomà, M., Alemany, L., Bosch Jose, F.X. & Pavón, M.A. (2022). The Isothermal Amplification AmpFire Assay for Human Papillomavirus (HPV) Detection and Genotyping in Formalin-Fixed, Paraffin-Embedded Oropharyngeal Cancer Samples. Journal of Molecular Diagnostics, 24(1), 79-87. doi: 10.1016/j.jmoldx.2021.10.008-
dc.identifier.issn1525-1578MIAR
-
dc.identifier.urihttp://hdl.handle.net/10609/146665-
dc.description.abstractHuman papillomavirus (HPV)-related oropharyngeal squamous cell carcinomas (OPSCCs) represent a distinct clinical entity compared with HPV-negative tumors with particular regard to treatment response and survival outcome. The aim of this study was to assess the AmpFire Multiplex HR-HPV tests, for the detection and genotyping of 15 high-risk (HR) HPV types in formalin-fixed, paraffin-embedded (FFPE) samples and identify HPV-driven OPSCC. DNA from 160 OPSCC FFPE specimens plus 23 samples from other head and neck primary sites was tested. Results were compared with those obtained using Linear Array HPV-DNA Genotyping Test. Linear Array and AmpFire Multiplex HR-HPV tests showed, for all samples and specifically for OPSCCs, an overall concordance agreement of 98.9% and 99.4% and a Cohen ¿ coefficient of 0.972 and 0.984, respectively. A concordance of 100% for HPV16 and HPV18 was observed. The overall agreement between p16INK4a overexpression and HPV detection by the AmpFire Multiplex HR-HPV assay in 145 OPSCC samples was 93.8%, with a Cohen ¿ coefficient of 0.848. The AmpFire HPV Tests are simple assays for detection and genotyping of HPV-DNA in OPSCC FFPE samples and can be easily implemented in the clinical practice setting for HPV-DNA detection.en
dc.format.mimetypeapplication/pdf-
dc.language.isoengca
dc.publisherJournal of Molecular Diagnosticsca
dc.relation.ispartofJournal of Molecular Diagnostics, 2022, 24(1)-
dc.relation.ispartofseries24;1-
dc.relation.urihttps://doi.org/10.1016/j.jmoldx.2021.10.008-
dc.rightsCC BY-NC-ND 4.0-
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0-
dc.subjectHPV-DNA testen
dc.subjecthuman Papillomavirusen
dc.subjectoropharyngeal canceren
dc.subjectFFPEen
dc.subjectisothermal amplificationen
dc.subjectp16 (ink4a)en
dc.subjectprueba de ADN-VPHes
dc.subjectvirus del papiloma humanoes
dc.subjectcáncer de orofaringees
dc.subjectFFPEes
dc.subjectamplificación isotérmicaes
dc.subjectp16 (ink4a)es
dc.subjecttest de VPH-ADNca
dc.subjectvirus del papil·loma humàca
dc.subjectcàncer orofaríngeca
dc.subjectFFPEca
dc.subjectp16 (ink4a)ca
dc.subjectisotèrmic amplificacióca
dc.subject.lcshpapillomavirusesen
dc.titleThe Isothermal Amplification AmpFire Assay for Human Papillomavirus (HPV) Detection and Genotyping in Formalin-Fixed, Paraffin-Embedded Oropharyngeal Cancer Samplesca
dc.typeinfo:eu-repo/semantics/articleca
dc.subject.lemacpapil·lomavirusca
dc.subject.lcshespapilomaviruses
dc.rights.accessRightsinfo:eu-repo/semantics/embargoedAccess-
dc.identifier.doihttp://doi.org/10.1016/j.jmoldx.2021.10.008-
dc.gir.idAR/0000009421-
dc.relation.projectIDinfo:eu-repo/grantAgreement/AGAUR/SGR/2017SGR1085-
dc.relation.projectIDinfo:eu-repo/grantAgreement/CIBERESP/CB06/02/0073-
dc.type.versioninfo:eu-repo/semantics/acceptedVersion-
dc.date.embargoEndDate2022-11-17-
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