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Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Paytubi, Sonia | - |
dc.contributor.author | Taberna , Miren | - |
dc.contributor.author | Mena, Marisa | - |
dc.contributor.author | Quirós, Beatriz | - |
dc.contributor.author | Gomà-i-Freixanet, Montserrat | - |
dc.contributor.author | Alemany, Laia | - |
dc.contributor.author | BOSCH JOSÉ, FRANCESC XAVIER | - |
dc.contributor.author | PAVÓN, MIGUEL ANGEL | - |
dc.contributor.other | Institut Català d'Oncologia | - |
dc.contributor.other | Institut d'Investigació Biomèdica de Bellvitge | - |
dc.contributor.other | Instituto de Salud Carlos III | - |
dc.contributor.other | Hospital Universitari de Bellvitge | - |
dc.contributor.other | Universitat Oberta de Catalunya | - |
dc.date.accessioned | 2022-08-09T06:58:06Z | - |
dc.date.available | 2022-08-09T06:58:06Z | - |
dc.date.issued | 2021-11-17 | - |
dc.identifier.citation | Paytubi, S., Taberna, M., Quirós, B., Gomà, M., Alemany, L., Bosch Jose, F.X. & Pavón, M.A. (2022). The Isothermal Amplification AmpFire Assay for Human Papillomavirus (HPV) Detection and Genotyping in Formalin-Fixed, Paraffin-Embedded Oropharyngeal Cancer Samples. Journal of Molecular Diagnostics, 24(1), 79-87. doi: 10.1016/j.jmoldx.2021.10.008 | - |
dc.identifier.issn | 1525-1578MIAR | - |
dc.identifier.uri | http://hdl.handle.net/10609/146665 | - |
dc.description.abstract | Human papillomavirus (HPV)-related oropharyngeal squamous cell carcinomas (OPSCCs) represent a distinct clinical entity compared with HPV-negative tumors with particular regard to treatment response and survival outcome. The aim of this study was to assess the AmpFire Multiplex HR-HPV tests, for the detection and genotyping of 15 high-risk (HR) HPV types in formalin-fixed, paraffin-embedded (FFPE) samples and identify HPV-driven OPSCC. DNA from 160 OPSCC FFPE specimens plus 23 samples from other head and neck primary sites was tested. Results were compared with those obtained using Linear Array HPV-DNA Genotyping Test. Linear Array and AmpFire Multiplex HR-HPV tests showed, for all samples and specifically for OPSCCs, an overall concordance agreement of 98.9% and 99.4% and a Cohen ¿ coefficient of 0.972 and 0.984, respectively. A concordance of 100% for HPV16 and HPV18 was observed. The overall agreement between p16INK4a overexpression and HPV detection by the AmpFire Multiplex HR-HPV assay in 145 OPSCC samples was 93.8%, with a Cohen ¿ coefficient of 0.848. The AmpFire HPV Tests are simple assays for detection and genotyping of HPV-DNA in OPSCC FFPE samples and can be easily implemented in the clinical practice setting for HPV-DNA detection. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | ca |
dc.publisher | Journal of Molecular Diagnostics | ca |
dc.relation.ispartof | Journal of Molecular Diagnostics, 2022, 24(1) | - |
dc.relation.ispartofseries | 24;1 | - |
dc.relation.uri | https://doi.org/10.1016/j.jmoldx.2021.10.008 | - |
dc.rights | CC BY-NC-ND 4.0 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0 | - |
dc.subject | HPV-DNA test | en |
dc.subject | human Papillomavirus | en |
dc.subject | oropharyngeal cancer | en |
dc.subject | FFPE | en |
dc.subject | isothermal amplification | en |
dc.subject | p16 (ink4a) | en |
dc.subject | prueba de ADN-VPH | es |
dc.subject | virus del papiloma humano | es |
dc.subject | cáncer de orofaringe | es |
dc.subject | FFPE | es |
dc.subject | amplificación isotérmica | es |
dc.subject | p16 (ink4a) | es |
dc.subject | test de VPH-ADN | ca |
dc.subject | virus del papil·loma humà | ca |
dc.subject | càncer orofarínge | ca |
dc.subject | FFPE | ca |
dc.subject | p16 (ink4a) | ca |
dc.subject | isotèrmic amplificació | ca |
dc.subject.lcsh | papillomaviruses | en |
dc.title | The Isothermal Amplification AmpFire Assay for Human Papillomavirus (HPV) Detection and Genotyping in Formalin-Fixed, Paraffin-Embedded Oropharyngeal Cancer Samples | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.subject.lemac | papil·lomavirus | ca |
dc.subject.lcshes | papilomavirus | es |
dc.rights.accessRights | info:eu-repo/semantics/embargoedAccess | - |
dc.identifier.doi | http://doi.org/10.1016/j.jmoldx.2021.10.008 | - |
dc.gir.id | AR/0000009421 | - |
dc.relation.projectID | info:eu-repo/grantAgreement/AGAUR/SGR/2017SGR1085 | - |
dc.relation.projectID | info:eu-repo/grantAgreement/CIBERESP/CB06/02/0073 | - |
dc.type.version | info:eu-repo/semantics/acceptedVersion | - |
dc.date.embargoEndDate | 2022-11-17 | - |
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