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Title: Análisis transcriptómico asociado a la producción de betacaroteno en Yuca
Author: Ovalle Rivera, Tatiana Melissa
Tutor: Pantano Rubiño, Lorena
Others: Marco-Galindo, Maria-Jesús  
Keywords: RNA-seq
Issue Date: 13-Jun-2018
Publisher: Universitat Oberta de Catalunya (UOC)
Abstract: Malnutrition affects thousands of millions of people in the world, because of critical crops to food security contain low levels of micronutrients. Cassava (Manihot esculenta Crantz) is the staple food of more than 500 million people in the world and the breeding programs have been focused in increase the ¿-carotene level in roots. To support the breeding processes, this research aimed to identify differential expression in genotypes with different levels of ¿-carotene in roots and understand what genome proportion is activated during high production of this compound. To accomplish this goal, six genotypes from a segregating family and two parental were chosen to conduct RNA sequencing. The bioinformatics pipeline for this research included: HISAT for mapping, StringTie for transcriptome assembly, Kallisto for expression quantification, DESeq2 for differential expression analysis and DEGreport to obtain a graphical view of the results. The annotation, function and regulation was obtained from PlantRegMap. The results showed that over expressed genome regions during ¿-carotene accumulation are associated to heat stress, temperature and luminous intensity, when there is no ¿-carotene accumulation, there are other activated synthesis pathways, mainly the sterole synthesis. Additionally, three regulator genes were identify in the root transcriptome and 42 genes in leaf transcriptome. The impact of the information from this research has several implications for the Cassava Breeding Program and improve the comprehension of the ¿-carotene accumulation in cassava roots.
Language: Spanish
Appears in Collections:Trabajos finales de carrera, trabajos de investigación, etc.

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