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Title: | Análisis filogenético de la óxido nítrico reductasa (Nor): establecimiento del perfil de la proteína |
Author: | Valverde Guirao, Ana Belén |
Tutor: | Pizarro Tobías, Paloma |
Others: | Perez-Navarro, Antoni |
Abstract: | This project aimed to carry out a phylogenetic analysis and the development of the profile of the nitric oxide reductase (Nor). This enzyme contributes to the production of nitrous oxide (N2O), which is a powerful greenhouse gas, and it participates in Ozone Depletion. The starting point of the study was the obtaining of a database of bacterial Nor proteins through PSI-BLAST. The methodology carried out was based on the use of different bioinformatics frameworks. They were used software such as MEGAX, ModelTest or TREE-PUZZLE for the phylogenetic analysis, and HMMER for the development of the profile. In addition, R was used for the creation of small programs that helped to continue with the study, among other programs. The results showed that the tree organized the sequences in two different clades. In one of them, bacteria of the Rhizobiaceae family predominated, while in the other, the majority were of the order Rhodobacterales. On the other hand, it was found that the sequence-based profiles were able to identify proteins annotated as Nor in a protein database and proteins denoted as unclassified, as well. The results suggested that the profiles could be used in the future to study the role of Nor in increasing levels of atmospheric N2O. In this way, different approaches could be carried out to mitigate this serious environmental problem. |
Keywords: | nitric oxide reductase phylogenetic analysis protein profile òxid nítric reductasa |
Document type: | info:eu-repo/semantics/masterThesis |
Issue Date: | Jun-2021 |
Publication license: | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
Appears in Collections: | Trabajos finales de carrera, trabajos de investigación, etc. |
Files in This Item:
File | Description | Size | Format | |
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avalverdeguiTFM0621memoria.pdf | Memoria del TFM | 1,68 MB | Adobe PDF | View/Open |
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