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http://hdl.handle.net/10609/81671
Title: | Desarrollo de un proceso de análisis de datos RNA-seq, para el estudio de la expresión diferencial en distintos puntos en el tiempo |
Author: | Magallón Lorenz, Miriam |
Tutor: | Gel Moreno, Bernat |
Others: | Universitat Oberta de Catalunya Ventura, Carles |
Abstract: | The aim of this project was to develop an analysis process for RNA-seq data to study the differentiation of Schwann cells between time points. We implement several functions using R and Bioconductor packages, such as DESeq2. We did it taking as reference the biological questions researchers have, in order to answer it as properly as possible. Regarding to the implemented functions, we get some of them capable of analysing differential expressed genes, as well as, others to show specific gene expression over time, giving the user more flexibility to answer specific researcher¿s questions. In order to check this developed methodology, we analyse fibroblast samples (FiPS), which were used as control for the whole analysis. We prove that the differentiation process was taken place as expected. In addition, we found new possible markers for the different stages of differentiation process, using implemented functions. In conclusion, the several functions implemented worked to answer researcher questions properly. |
Keywords: | DESeq2 RNA-seq data analysis |
Document type: | info:eu-repo/semantics/masterThesis |
Issue Date: | 5-Jun-2018 |
Publication license: | http://creativecommons.org/licenses/by-sa/3.0/es/ |
Appears in Collections: | Trabajos finales de carrera, trabajos de investigación, etc. |
Files in This Item:
File | Description | Size | Format | |
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mmagaloTFM0618memoria.pdf | Memoria del TFM | 2,95 MB | Adobe PDF | View/Open |
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