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Title: Comparative study of bacterial and fungal alpha-amylase industrial producers
Author: Torrents Soler, Maria
Director: Merino Arranz, David
Ventura Royo, Carles  
Tutor: Pizarro Tobías, Paloma
Keywords: alfa-amilasa
Bacillus licheniformis
Aspergillus oryzae
Issue Date: 2-Jan-2019
Publisher: Universitat Oberta de Catalunya (UOC)
Abstract: There are several industrial sectors that use enzymes from microorganisms to generate products for a wide range of applications. Among all industrial enzymes, alpha amylase is one of the main enzymes used in industry. Furthermore, microbial enzymes have gained interest for their widespread uses in industries and medicine owing to their easy availability, stability, catalytic activity, ease of production and optimization than plant and animal enzymes, hence they are the favored sources for industrial enzymes production. The extensive applications and the broad utility variety of microbial enzymes arises the interest of a comparative study of different alpha-amylase microbial producers along with a structure and sequence based analysis of alpha-amylase protein from Bacillus licheniformis and Aspergillus oryzae, likewise, they are the most utilized industrial bacterial and fungal alpha-amylase producers. Phylogenetic analysis pretends to study the evolutive and structural relationship between some Bacillus spp alpha-amylase producers and Aspergillus spp alpha-amylase producers. Also, its goal is to perform a comparison between natural producers and the industrially ones, in addition to study the evolutionary relationships within the spices. On the basis of the establish evolutionary relationships Bacillus paralicheniformis and Bacillus halotolerans show potential as new alpha-amylase industrial producers. Comparative genomic study between the coding alpha-amylase gene obtained from the respective protein and the microbial genome aims to provide a highly detailed view of the protein genetics.Protein profile analysis results in a structural and functional study of the protein, together with an homology alpha-amylase B.licheniformis and A.oryzae model.
Language: English
Appears in Collections:Bachelor thesis, research projects, etc.

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