Please use this identifier to cite or link to this item:
http://hdl.handle.net/10609/96346
Title: | Estudio de genómica comparativa de genes bacterianos relacionados con la ruta catabólica inferior del naftaleno: metabolismo del salicilato |
Author: | Zarandona Garai, Aitor |
Tutor: | Pizarro Tobías, Paloma |
Others: | Merino, David |
Abstract: | The intent of this document is to make a genomic comparative analysis of bacterial genes involved in the lower naphthalene catalytic pathway, the salicylate pathway. Naphthalene is one of the most pollutant polycyclic aromatic hydrocarbon (PAH). These components are hard to degrade but some bacteria, fungi and algae are able to metabolize aromatic compounds. This study focuses on salicylate hydroxylase enzyme (EC:1.14.13.1), which catalyzes the reaction from salicylate to catechol. Its coding sequence is found in the NAH7 plasmid of the Pseudomonas putida G7 strain, one of the most studied PAH metabolizing microorganisms. Starting from the aminoacidic chain a blast is made in order to find homologous proteins. Phylogenetic trees and multiple sequence alignments are also done in order to compare the preselected sequences. The final step consists in finding its genomic localization, whether it is part of an operon or not and making a comparative of the operons that are similar. BLAST, MEGAX, FGenesB and SnapGene are some of the bioinformatics tools used to reach the objectives. The results obtained from the phylogenetic trees, database and sequence comparison lead to a conclusion, the gene that codes the salicylate hydroxylase enzyme is not part of a highly conserved operon. |
Keywords: | biodegradation genomic compared naphthalene |
Document type: | info:eu-repo/semantics/masterThesis |
Issue Date: | 4-Jun-2019 |
Publication license: | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
Appears in Collections: | Trabajos finales de carrera, trabajos de investigación, etc. |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
zaran1905TFM0619memoria.pdf | Memoria del TFM | 2,38 MB | Adobe PDF | View/Open |
Share:
This item is licensed under a Creative Commons License