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http://hdl.handle.net/10609/122746
Título : | Identifying crossing collagen fibers in human corneal tissues using pSHG images |
Autoría: | Alizadeh, M. Merino, David Lombardo, Giuseppe Lombardo, Marco Mencucci, Rita Ghotbi, M. Loza Alvarez, Pablo |
Otros: | University of Kurdistan Istituto per i Processi Chimico-Fisici Università degli Studi di Firenze Institut de Ciències Fotòniques Universitat Oberta de Catalunya (UOC) |
Citación : | Alizadeh, M., Merino, D., Lombardo, G., Lombardo, M., Mencucci, R., Ghotbi, M. & Loza-Alvarez, P. (2019). Identifying crossing collagen fibers in human corneal tissues using pSHG images. Biomedical Optics Express, 10(8), 3875-3888. doi: 10.1364/BOE.10.003875 |
Resumen : | Polarization sensitive second harmonic generation (pSHG) microscopy has been used previously to characterize the structure of collagen fibers in corneal samples. Due to the typical organization of the corneal stroma, the information that pSHG provides may be misleading in points where two different collagen fiber bundles orient along different direction crossings. Here, a simulation that illustrates the problem is presented, along with a novel method that is capable of identifying these crossing points. These results can be used to improve the evaluation of corneal collagen structure, and it has been applied to analyze pSHG data acquired from healthy and keratoconic human corneal samples. |
Palabras clave : | imágenes de pSHG córnea estructura de colágeno |
DOI: | 10.1364/BOE.10.003875 |
Tipo de documento: | info:eu-repo/semantics/article |
Versión del documento: | info:eu-repo/semantics/publishedVersion |
Fecha de publicación : | 1-ago-2019 |
Licencia de publicación: | http://creativecommons.org/licenses/by/3.0/es/ |
Aparece en las colecciones: | Articles cientÍfics Articles |
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Merino_BOE_Identifying.pdf | 6,5 MB | Adobe PDF | Visualizar/Abrir |
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