Please use this identifier to cite or link to this item:
http://hdl.handle.net/10609/134326
Title: | Leveraging single-cell ATAC-seq data to gain insights into the cell-type selective component of the human pancreatic islet regulome |
Author: | Castillo Bonilla, Andrés |
Tutor: | Garrido Martín, Diego Ferrer, Jorge |
Others: | Calvet Liñán, Laura |
Abstract: | ATAC-seq is essential for profiling chromatin accessibility and characterizing the transcriptional regulatory landscape. However, the recent shift towards the study of heterogeneous cell populations poses a challenge for bulk ATAC-seq. Thus, single-cell ATAC-seq has emerged as a response to the limitations of bulk ATACseq when studying cellular heterogeneity. We aim to to characterize the cell-typeselective component of enhancers using scATAC-seq data. To achieve this purpose, we a) annotate regulome signatures across cell-type selective open chromatin regions, b) estimate TF motif enrichment among cell-type selective enhancers, c) detect accessible cell-type selective enhancers that show robust TF binding and d) identify T2D-associated SNPs affecting TF binding across celltype selective enhancers. Motif enrichment analysis presented well-defined groups of TF motifs enriched across islet cell-type selective enhancers. TF motif occurrences across cell-type selective enhancers showed that enhancers bound by a given TF was consistent with the cell-type selective clustering observed in the TF motif enrichment analysis. Finally, the integration of TF-binding characterizing islet cell-type enhancers with fine-mapped T2D genetic variants allowed us to propose the most likely molecular mechanism underlying a few T2D risk loci. |
Keywords: | human pancreatic islet genome regulation genetic |
Document type: | info:eu-repo/semantics/masterThesis |
Issue Date: | Jun-2021 |
Publication license: | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
Appears in Collections: | Trabajos finales de carrera, trabajos de investigación, etc. |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
acastilloboTFM0621memoria.pdf | Memoria del TFM | 1,27 MB | Adobe PDF | View/Open |
Share:
This item is licensed under a Creative Commons License