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http://hdl.handle.net/10609/146665
Título : | The Isothermal Amplification AmpFire Assay for Human Papillomavirus (HPV) Detection and Genotyping in Formalin-Fixed, Paraffin-Embedded Oropharyngeal Cancer Samples |
Autoría: | Paytubi, Sonia Taberna , Miren Mena, Marisa Quirós, Beatriz Gomà-i-Freixanet, Montserrat Alemany, Laia Bosch José, Francesc Xavier Pavón, Miguel Ángeñ |
Otros: | Institut Català d'Oncologia Institut d'Investigació Biomèdica de Bellvitge Instituto de Salud Carlos III Hospital Universitari de Bellvitge Universitat Oberta de Catalunya |
Citación : | Paytubi, S., Taberna, M., Quirós, B., Gomà, M., Alemany, L., Bosch Jose, F.X. & Pavón, M.A. (2022). The Isothermal Amplification AmpFire Assay for Human Papillomavirus (HPV) Detection and Genotyping in Formalin-Fixed, Paraffin-Embedded Oropharyngeal Cancer Samples. Journal of Molecular Diagnostics, 24(1), 79-87. doi: 10.1016/j.jmoldx.2021.10.008 |
Resumen : | Human papillomavirus (HPV)-related oropharyngeal squamous cell carcinomas (OPSCCs) represent a distinct clinical entity compared with HPV-negative tumors with particular regard to treatment response and survival outcome. The aim of this study was to assess the AmpFire Multiplex HR-HPV tests, for the detection and genotyping of 15 high-risk (HR) HPV types in formalin-fixed, paraffin-embedded (FFPE) samples and identify HPV-driven OPSCC. DNA from 160 OPSCC FFPE specimens plus 23 samples from other head and neck primary sites was tested. Results were compared with those obtained using Linear Array HPV-DNA Genotyping Test. Linear Array and AmpFire Multiplex HR-HPV tests showed, for all samples and specifically for OPSCCs, an overall concordance agreement of 98.9% and 99.4% and a Cohen ¿ coefficient of 0.972 and 0.984, respectively. A concordance of 100% for HPV16 and HPV18 was observed. The overall agreement between p16INK4a overexpression and HPV detection by the AmpFire Multiplex HR-HPV assay in 145 OPSCC samples was 93.8%, with a Cohen ¿ coefficient of 0.848. The AmpFire HPV Tests are simple assays for detection and genotyping of HPV-DNA in OPSCC FFPE samples and can be easily implemented in the clinical practice setting for HPV-DNA detection. |
Palabras clave : | prueba de ADN-VPH virus del papiloma humano cáncer de orofaringe FFPE amplificación isotérmica p16 (ink4a) |
DOI: | http://doi.org/10.1016/j.jmoldx.2021.10.008 |
Tipo de documento: | info:eu-repo/semantics/article |
Versión del documento: | info:eu-repo/semantics/acceptedVersion |
Fecha de publicación : | 17-nov-2021 |
Licencia de publicación: | http://creativecommons.org/licenses/by-nc-nd/4.0 |
Aparece en las colecciones: | Articles Articles cientÍfics |
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Fichero | Descripción | Tamaño | Formato | |
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Paytubi_ JMDI_isothermal.pdf | 855,5 kB | Adobe PDF | Visualizar/Abrir |
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