Please use this identifier to cite or link to this item: http://hdl.handle.net/10609/146665
Title: The Isothermal Amplification AmpFire Assay for Human Papillomavirus (HPV) Detection and Genotyping in Formalin-Fixed, Paraffin-Embedded Oropharyngeal Cancer Samples
Author: Paytubi, Sonia
Taberna , Miren
Mena, Marisa  
Quirós, Beatriz
Gomà-i-Freixanet, Montserrat  
Alemany, Laia
BOSCH JOSÉ, FRANCESC XAVIER  
PAVÓN, MIGUEL ANGEL  
Others: Institut Català d'Oncologia
Institut d'Investigació Biomèdica de Bellvitge (IDIBELL)
Instituto de Salud Carlos III
Hospital Universitari de Bellvitge
Universitat Oberta de Catalunya
Keywords: HPV-DNA test
human Papillomavirus
oropharyngeal cancer
FFPE
isothermal amplification
p16 (ink4a)
Issue Date: 17-Nov-2021
Publisher: Journal of Molecular Diagnostics
Citation: Paytubi, S., Taberna, M., Quirós, B., Gomà, M., Alemany, L., Bosch Jose, F.X. & Pavón, M.A. (2022). The Isothermal Amplification AmpFire Assay for Human Papillomavirus (HPV) Detection and Genotyping in Formalin-Fixed, Paraffin-Embedded Oropharyngeal Cancer Samples. Journal of Molecular Diagnostics, 24(1), 79-87. doi: 10.1016/j.jmoldx.2021.10.008
Published in: 24;1
Project identifier: info:eu-repo/grantAgreement/AGAUR/SGR/2017SGR1085
info:eu-repo/grantAgreement/CIBERESP/CB06/02/0073
Also see: https://doi.org/10.1016/j.jmoldx.2021.10.008
Abstract: Human papillomavirus (HPV)-related oropharyngeal squamous cell carcinomas (OPSCCs) represent a distinct clinical entity compared with HPV-negative tumors with particular regard to treatment response and survival outcome. The aim of this study was to assess the AmpFire Multiplex HR-HPV tests, for the detection and genotyping of 15 high-risk (HR) HPV types in formalin-fixed, paraffin-embedded (FFPE) samples and identify HPV-driven OPSCC. DNA from 160 OPSCC FFPE specimens plus 23 samples from other head and neck primary sites was tested. Results were compared with those obtained using Linear Array HPV-DNA Genotyping Test. Linear Array and AmpFire Multiplex HR-HPV tests showed, for all samples and specifically for OPSCCs, an overall concordance agreement of 98.9% and 99.4% and a Cohen ¿ coefficient of 0.972 and 0.984, respectively. A concordance of 100% for HPV16 and HPV18 was observed. The overall agreement between p16INK4a overexpression and HPV detection by the AmpFire Multiplex HR-HPV assay in 145 OPSCC samples was 93.8%, with a Cohen ¿ coefficient of 0.848. The AmpFire HPV Tests are simple assays for detection and genotyping of HPV-DNA in OPSCC FFPE samples and can be easily implemented in the clinical practice setting for HPV-DNA detection.
Language: English
URI: http://hdl.handle.net/10609/146665
ISSN: 1525-1578MIAR
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