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http://hdl.handle.net/10609/146986
Títol: | Molecular imprinting as a tool for determining molecular markers: a lung cancer case |
Autoria: | Piletska, Elena Magumba, Kirabo Joseph, Lesslly Garcia Cruz, Álvaro Norman, Rachel Singh, Rajinder Tabasso, Antonella F. S. Jones, Donald Macip, Salvador Piletsky, Sergey |
Altres: | University of Leicester Universitat Oberta de Catalunya (UOC) |
Citació: | Piletska, E., Magumba, K., Joseph, L., Garcia Cruz, A., Norman, R., Singh, R., Tabasso, A., Jones, D.J.L., Macip, S. & Piletsky, S.A. (2022). Molecular imprinting as a tool for determining molecular markers: a lung cancer case. RSC Advances, 12(28), 17747-17754. doi: 10.1039/d2ra01830f |
Resum: | Determining which cancer patients will be sensitive to a given therapy is essential for personalised medicine. Thus, it is important to develop new tools that will allow us to stratify patients according to their predicted response to treatment. The aim of work presented here was to use molecular imprinting for determining the sensitivity of lung cancer cell lines to ionising radiation based on cell surface proteomic differences. Molecularly imprinted polymer nanoparticles (nanoMIPs) were formed in the presence of whole cells. Following trypsinolysis, protein epitopes protected by complexing with MIPs were eluted from the nanoparticles and analysed by LC-MS/MS. The analysis identified two membrane proteins, neutral amino acid transporter B (0) and 4F2 cell-surface antigen heavy chain, the abundance of which in the lung cancer cells could indicate resistance of these cells to radiotherapy. This proof-of-principle experiments shows that this technology can be used in the discovery of new biomarkers and in development of novel diagnostic and therapeutic tools for a personalised medicine approach to treating cancer. |
Paraules clau: | proteinas cancer de pulmons macardors moleculars proteines |
DOI: | https://www.doi.org/10.1039/d2ra01830f |
Tipus de document: | info:eu-repo/semantics/article |
Versió del document: | info:eu-repo/semantics/publishedVersion |
Data de publicació: | 15-jun-2022 |
Llicència de publicació: | http://creativecommons.org/licenses/by-nc/4.0 |
Apareix a les col·leccions: | Articles Articles cientÍfics |
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Molecular imprinting as a tool for determining molecular markers a lung cancer case.pdf | Molecular imprinting as a tool for determining molecular markers a lung cancer case | 465,26 kB | Adobe PDF | Veure/Obrir |
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